No. One & Only should never be frozen, nor should it be kept at temperatures above 95°F. Normal room temperature will not harm the bacteria in One & Only. Perhaps it is easier to think about it this way: One & Only is not like an ice cream cone. Once ice cream is exposed to room temperatures it starts to melt, and if it completely melts you have a mess and no longer ice cream. One & Only is not like this. One & Only will last for 6 months at room temperatures (65 to 85°F). At colder temperatures (around 50°F) One & Only will last one year.